To investigate whether D-mannose anti-obesity like effect is depended on adipose ACSS2, we injected the AAV9 vectors encoding adiponectin-drived small RNA against Acss2 to down-regulate adipose ACSS2 expression in D-mannose-administrated HFD-treated mice (Fig. S9A–C). The gene discussed is ACSS2; the disease is obesity due to melanocortin 4 receptor deficiency.