ACSS2 and obesity due to melanocortin 4 receptor deficiency: To further investigate adipose-specific ACSS2 function, we generated adipose-specific ACSS2 over-expression mice (AAV-ACSS2) to evaluate its phenotype with the high fat diet (HFD)-induced obesity by injected the AAV9 vectors encoding adiponectin-drived ACSS2 over-expression in wild type mice (Fig. 6A).