Further dimensionality reduction and clustering were applied to the major cell populations, and this analysis revealed distinct sub-populations within the major cell types, including 9 sub-populations of CD4 + T cells, 6 sub-populations of CD8 + T cells, 5 sub-populations of NK cells, 3 sub-populations of dendritic cells, 9 sub-populations of macrophages, 2 sub-populations of mast cells, 9 sub-populations of B cells, 8 sub-populations of fibroblasts, 6 sub-populations of endothelial cells, and 20 sub-populations of tumor cells (Fig. 1d, e and Supplementary Fig. 2). This evidence concerns the gene CD8A and neoplasm.