To corroborate these findings in a more physiological cell model, we used human osteosarcoma U2OS cells in which we measured PTHR1-mediated cAMP accumulation with the fluorescence resonance energy transfer (FRET)-based cAMP biosensor Epac-S-H187 (Klarenbeek et al, 2015), in the absence or presence of ADAM19. Here, PTH1R is linked to osteosarcoma.