Thus, ex vivo whole-mount tumor confocal microscopy and 3D reconstruction of confocal images following in situ fluorescence labeling of blood vessels (i.v. administration of anti-PECAM-1 antibody), confirmed a robust vascular disruption and decreased invasion depth of tumor vessels (10–30 versus 70–90 μm, respectively) in PMNlo compared with PMNhi tumors (Figure 4, D and E). Here, PECAM1 is linked to neoplasm.