To avoid systemic effects of Pfkfb3 overexpression we studied cultured L6-GLUT4-HA myotubes, which display robust insulin regulation of GLUT4 trafficking and develop insulin resistance upon palmitate treatment, mimicking lipotoxicity, a trigger of in vivo insulin resistance (Hoehn et al., 2009). Here, PFKFB3 is linked to Insulin resistance.