Four weeks after bilateral infusion of the AAV-CaMKII-hChR2-mCherry vector (AAV-ChR2, n = 6) or a control vector AAV-CaMKII-mCherry (AAV-mCherry, n = 6) into the rat dmPFC, we found that the AAV-ChR2- and AAV-mCherry-injected rats displayed a similar level of anxiety-like behavior as measured by the open field test (OFT) during the initial 5-min period without stimulation (light off) (time spent in central zone or central time: control, 13.3 ± 5.4 s, ChR2, 13.2 ± 3.4 s, t = 0.052, p > 0.99, multiple comparisons of 2-way ANOVA). This evidence concerns the gene CAMK2G and Anxiety.