Therefore, we examined 15 pioneer TFs (83) and found that one (ASCL1) and two (CEBPA and GATA) pioneer TFs were upregulated in ApoE–/– at 6 weeks and 78 weeks, respectively; one (SOX2) pioneer TFs was upregulated in PPE-AAA; and one (GATA) and one (FOXA) pioneer TFs were upregulated in ApoE–/– Ang II-AAA at 7 days and 14 days, indicating that five pioneer TFs, including ASCL1, CEBPA, GATA, SOX2, and FOXA, play significant role in aortic diseases (Figure 5E). This evidence concerns the gene ASCL1 and triple-A syndrome.