More specifically, the expression of IRF2, NLRP1, NLRP6, PRKACA, PLCG1, ELANE, CASP3, and GZMA was increased, while the expression of IL6, IL1B, IL1A, CASP4, NLRP7, NLRP9, NLRP2, NAIP, CYCS, CASP6, CASP9 and GSDMB was decreased in NASH compared with normal tissues as shown in volcano plot and heatmap (Fig. 1B,C, p < 0.001). This evidence concerns the gene CASP6 and metabolic dysfunction-associated steatohepatitis.