Through Pearson correlation analysis in all samples, we detected the strongest relationship between SCAF11 and HMGB1 (R = 0.72, p < 0.001), while as in NASH samples, we found the strongest relationship between IL6 and IL1B (R = 0.7, p < 0.001), as shown in Supplementary Fig. S1A. These results indicated that expression imbalances of PRGs played important roles in the development of NASH. The gene discussed is HMGB1; the disease is metabolic dysfunction-associated steatohepatitis.