DSG2 and Moyamoya disease: To validate the biological role of DSG2 in Moyamoya disease, NC-pEGFP-C1 and DSG2-pEGFP-C1 plasmids were transfected into hCMEC/D3 cells to overexpress DSG2, and siRNA-NC and siRNA-DSG2 were transfected into HUVECs to knockdown DSG2.