Single-cell analysis revealed that MICAL2 was mainly concentrated in the fibroblasts of pancreatic cancer, while immunohistochemical staining indicated that MICAL2 expression was positively correlated with the expression of CAFs biomarkers, FAP and ACTA2, proving that MICAL2 was closely associated with CAFs in pancreatic cancer. Here, FAP is linked to familial pancreatic carcinoma.