RT-qPCR showed that AT3:VCP targets were more highly expressed in mouse AT3-initiated tumors treated with vehicle than in control mouse tumors (osteosarcoma, synovial sarcoma); CB-5083 for 4 days immediately prior to harvest also reduced expression of most of these (Fig. 8h, Supplementary Fig. 8h). The gene discussed is VCP; the disease is osteosarcoma.