Similar to the results obtained in a previous study in which organoids from healthy donors were analyzed,37 we found that the number of NeuN-positive and Myt1L-positive post-mitotic neurons was dramatically reduced in ALS patient-derived organoids after NMDA exposure, while GFAP expression remained unchanged (Figures 3D–3G). Here, RBFOX3 is linked to amyotrophic lateral sclerosis.