Meanwhile, immunoblotting assayed that cellular inflammation (p-p65) and pyroptosis-related proteins (NLRP3, ASC, caspase-1 p20, and GSDMD-N) were elevated in the lung tissues of ALI rats, while XBJ pretreatment could inhibit these proteins (Fig. 1 E, F). The gene discussed is NLRP3; the disease is acute respiratory distress syndrome.