In the hippocampus of female APP/PS1 AD mice, these signature genes included 13 candidates (Asic4, Pcsk1, Etnppl, Myh7b, Spag5, Gpr1, Myh8, Ccdc184, Pdzph1, Trim66, BC030499, Rims3, Prr16), only two candidates (LOC105247294, Ptpn6) in the RS cortex of female APP/PS1 AD mice, six gene candidates in the hippocampus of male APP/PS1 AD mice (4933407I18Rik, Hlcs, Hdac9, Pla2g4e, Wnt9a. The gene discussed is SPAG5; the disease is Alzheimer disease.