To assess the effect of AMCase and/or gastric acid in vivo, we pre-treated mice with the AMCase inhibitor 112252, omeprazole (a commercially available proton pump inhibitor), or 10% sodium bicarbonate by oral gavage once, 1 hour prior to infection with 2,500 Ascaris eggs and measured larval burden in the liver at 4 days post infection [15] (Fig 2D). This evidence concerns the gene CHIA and infection.