To further investigate the functions of PBX3 in glioma, we performed GSEA using TCGA data, GSEA showed that regulation of NOS1 pathway, CK1 pathway, HDAC pathway, Phosphatidylinositol system, Neurotransmitter receptor complex were differentially concentrated in PBX3 high expression phenotype. The gene discussed is PBX3; the disease is glioma.