To investigate this, we therefore cloned ACADM exon 2 and flanking intronic sequences into a splicing reporter vector with either the wild-type (WT) sequence or the sequence containing mutations identified by newborn screening to cause medium-chain acyl-CoA dehydrogenase deficiency (MCADD) (33) (Figure 1D). Here, ACADM is linked to medium chain acyl-CoA dehydrogenase deficiency.