TNF and neoplasm: To seek out specific SASP factors promoting CD73 expression, we performed qPCR to test cytokines expression in senescent LLC cells, including Il1α, Il1b, Il6, Il10, Ifnα, and Tnfα. The expression level of Il1α, Il6, Il10, and Tnfα were significantly upregulated in senescent tumor cells (Figure 3A).