For instance, in a rAAV5-based vector expressing FVIII in men with severe hemophilia A (NCT02576795) 87, exclusion was based on an in vitro transduction inhibition assay with a cut-off of 44.9% transduction and a total anti-AAV5 antibody assay cut-off of signal dilution ≥39.7%. Here, F8 is linked to hemophilia A.