Similarly, CD4+ T cells, CD8+ T cells, CD20+ B cells, CD45RO+ memory T cells and NCR1+ NK cells located in the tumor centre outside the TLS were significantly positively correlated with the density of TLSs in GNEN (ρ = 0.358, 0.613, 0.554, 0.507 and 0.254; P = 0.002, < 0.001, < 0.001, < 0.001 and = 0.034, respectively). The gene discussed is CD4; the disease is neoplasm.