HCRT and Anxiety: This is a novel finding that agrees with other findings, including our recent report in zebrafish showing that ablation of Hcrt neurons blocks anxiety behaviors induced by embryonic ethanol exposure24 and evidence in rodents showing that Hcrt injection decreases time spent in the light side of a light–dark test90 and chemogenetic and optogenetic stimulation of Hcrt neurons increases anxiety via social interaction45,91.