We investigated the role of S287 phosphorylation in tumor-promoting activity of PKM2 using PKM2 S287D and S287A mutants, and found that in H1299 cells in which endogenous PKM2 was silenced by shPKM2, re-expression of WT, S287D PKM2 and S287A PKM2 conferred approximately equal proliferation speed to the cells (Fig. 7g, h). Here, PKM is linked to neoplasm.