Further, we confirmed the results by flow cytometry, and the increased percentage of CD86+F4/80+ cells in CD11b+CD45+ cells and decreased percentage of CD206+F4/80+ cells in CD11b+CD45+ cells were observed in A2-APM group in GL261 and G422 tumor mice (Fig. 6c, Supplementary Fig. 43a and 44). The gene discussed is ITGAM; the disease is neoplasm.