The translocationof phosphatidylserine to the outer membrane leaflet induces the membranebudding and vesicle formation.37 Actin–myosininteractions cause the cytoskeletal structures to contract, concludingthis process.38 As per the literature,when checked in the melanoma model, overexpression of the GTP-bindingprotein ADP-ribosylation factor 6 (ARF6) leads to the increased productionof microvesicles.39 This evidence concerns the gene ARF6 and melanoma.