To determine the importance of the expression of PI3K/Akt/mTOR genes in the transmission of oncogenic signals that promote the growth and proliferation of DIPG, we analyzed a CRISPR/Cas9 loss-of-function screen data set performed on 38 DMG cell lines, representing all DMG H3 K27–altered subtypes (27). Here, PIK3CD is linked to diffuse intrinsic pontine glioma.