(2021) used CRISPR/Cas9 to establish transgenic zebrafish lines that express mutated NF1, Rb1 or TP53 under GFAP promoter and showed that various combinations of NF1, TP53, and/or Rb1 mutations can induce gliomas of different grades and phenotypes (108). The gene discussed is NF1; the disease is central nervous system cancer.