Interestingly, PDL1 or also known as CD274, which plays a crucial role in the suppression of T and NK cell activity [43,44], is only significantly upregulated in the triculture containing GBM, astrocytes and microglia displaying a 1.64 fold upregulation, indicating the importance of both stroma cell types and the beneficiary effect of this triculture. This evidence concerns the gene CD274 and glioblastoma.