As EMT play an important role in mediating cancer cell stemness, we first evaluated if EMT was regulated in the PARD3-driven hepatocarcinogenesis, Nonetheless, through a re-evaluation of the histological sections obtained from orthotopic tumours in both wild type and PARD3 overexpressing models, we observed that overexpression of PARD3 had marginal effects on the expression of epithelial marker E-cadherin and mesenchymal markers including N-cadherin and vimentin (Fig. S5A). This evidence concerns the gene CDH2 and cancer.