PARD3 and cancer: To comprehensively investigate the effects of PARD3 overexpression on the developmental and functional dynamics of early tumour ecosystems, we collected 21,294 cells from early-stage orthotopic murine liver tumours derived from wild-type and PARD3-overexpressing Hepa1-6 cells and subjected these cells to scRNA-seq. We identified 15 distinctive cell clusters containing immune, stromal and cancer cells.