To investigate the molecular mechanism of sunitinib resistance and Twist overexpression on the Wnt/β-catenin pathway and EMT in renal cell carcinoma, we performed Western blot analysis to identify the differentially expressed proteins among SR-A498 cells, oe-Twist A498 cells, oe-Twist SR-A498 cells, and control cells. The gene discussed is TWIST1; the disease is hereditary clear cell renal cell carcinoma.