To further confirm the identity of TMEM16A-expressing cells, we genetically deleted TMEM16A in ICC (TMEM16AICCKO) or in SMCs (TMEM16ASMKO) by crossing TMEM16Aflox/flox mice with KitCreERT2 mice20,36 or with SMACre mice20,31, respectively. The gene discussed is ANO1; the disease is intrahepatic cholangiocarcinoma.