To illustrate the experimental opportunities enabled by designing sLCRs to represent multiple cell states, we performed genome-wide pooled CRISPR activation screens (CRISPRa) in Isocitrate dehydrogenase wildtype human glioma-initiating cells (IDH-wildtype-hGICs) with either MGT1, MGT4 or PNGT3 as a readout. Here, IDH3A is linked to glioma.