Meanwhile, miR-4483 was decreased in fibrotic kidneys and HG or TGF-β1-treated HK-2 cells, whereas TIMP1 was inhibited by miR-4483 at both mRNA and protein levels, which may be the underlying mechanism that miR-4483 is involved in renal fibrosis, which requires further research. The gene discussed is TIMP1; the disease is renal fibrosis.