As shown in Fig. 3A, B, qRT-PCR results revealed that the mRNA expression levels of Fibronectin (FN), Collagen I (COL1), Collagen III (COL3), and a-SMA in 16w, 24w db/db mice and the FSGS group were remarkably higher than those in db/m mice of the same week and the control mice. This evidence concerns the gene FN1 and focal segmental glomerulosclerosis.