Finally, the protein–protein interaction network (PPI), GO, and KEGG enrichment were used to predict the top 10 significant target genes of MMP13 and TIMP1, which indicate that MMP13 and TIMP1 are closely related to the synthesis of extracellular matrix and collagen and thus participating in the process of renal fibrosis (Fig. 7I–N). This evidence concerns the gene MMP13 and renal fibrosis.