To determine the role of renal PSTPIP2 levels in AAI-induced AAN, we established a Pstpip2 conditional knock-in (Pstpip2-cKI) mouse model by CRISPR/Cas-mediated genome engineering (Figure 2A); PSTPIP2 was overexpressed in the RTECs. Here, PSTPIP2 is linked to Balkan nephropathy.