Considering each sample timepoint separately to avoid inflating genetic-distance-based diversity calculations in prolonged infections, we found higher intra-host diversity in PWH with CD4 counts <200 cells/μL than in PWH with higher CD4 counts or in PWOH as measured by normalized Shannon entropy, average pairwise genetic distance, and total numbers of haplotypes detected (Fig. 1b). This evidence concerns the gene CD4 and infection.