MKI67 and neoplasm: Another study detected the nuclear antigen (Ki67) of proliferating cells in tumor tissues by immunohistochemistry and found fewer Ki67-positive cells and lower integrated optical density (IOD) of VEGF in BT474 tumor-bearing nude mice treated with Huaier compared with the control group, these in vivo experiments confirme that Huaier could inhibit the breast cancer cells proliferation (Liu, 2016).