Another study detected the nuclear antigen (Ki67) of proliferating cells in tumor tissues by immunohistochemistry and found fewer Ki67-positive cells and lower integrated optical density (IOD) of VEGF in BT474 tumor-bearing nude mice treated with Huaier compared with the control group, these in vivo experiments confirme that Huaier could inhibit the breast cancer cells proliferation (Liu, 2016). This evidence concerns the gene MKI67 and breast carcinoma.