Animals treated with ADU-S100 + anti-ISG15 had a significant increase in the frequency of tumor infiltrating CD62L+CD44+ central memory CD8+ and CD4+ T cells compared to those treated with ADU-S100 alone, as well as an increase in CD44+CD62L- effector CD4+ TIL (Figure 6C, Supplementary Figure 6C). Here, CD4 is linked to neoplasm.