To further confirm the association between PSME3 and immune checkpoints, we conducted flow cytometry analysis after modulating PSME3 expression in 293T cells (Figure 7A) and silencing PSME3 in liver cancer Hut7, lung adenocarcinoma A549, and bladder cancer T24 cells (Figures 7B-D). This evidence concerns the gene PSME3 and lung adenocarcinoma.