TARDBP and proteostasis deficiencies: These results demonstrate that DCTN1 and other microtubule-associated motor proteins are modifiers that drive the aggregation of wild-type TDP-43 through the dysregulation of stress granule dynamics, indicating the crucial role of intracellular transport along microtubules in the pathological development of TDP-43 proteinopathies, including ALS/FTD.