To gain insights into the functional subtypes of T cell populations in CRC, we performed unsupervised cluster analysis using extracted T cells from all samples, including six subsets of CD4+T cells, five subsets of CD8+T cells, one NK cell subset, one mucosal associated invariant T cell subset (MAIT) and a proliferative T cell subset (Fig. 2A). Here, CD4 is linked to colorectal carcinoma.