Therefore, we applied Western blot assay to verify, and the results demonstrated that the expression of phosphorylated AKT (pAKT) in A375 cells and MV3 cell lines overexpressing DND1 decreased compared with the control group, but the total AKT expression did not exhibit a significant change, indicating that overexpression of DND1 downregulated the PI3K/AKT signaling pathway in melanoma cells. This evidence concerns the gene AKT1 and melanoma.