ATP plays a crucial role in providing energy for the active processes involved in EV biogenesis, including V-type proton ATPase subunit B (Atp6v1b2), RNA helicase DDX25 (Ddx25), and Sodium/potassium transporting ATPase subunits alpha-1 and 3 (Atp1a1, Atp1a3).32 ATP is also involved in cytoskeleton rearrangement processes required for EV biogenesis.33 Tumors express the ATP receptor P2X7 receptor (P2X7R) that contributes to EV biogenesis and secretion in tumor pathogenesis.34,35 GAPDH, a glycolytic enzyme, is associated with EV biogenesis, secretion, and assembly. The gene discussed is ATP1A1; the disease is neoplasm.