Further, we subjected these 64 DE-MRGs to univariate Cox regression analysis in breast cancer patients, revealing 9 genes (MRPL13, BCL2A1, ME3, SLC1A1, TP53AIP1, MTHFD2, ALDH2, ACSL1, TYMP) significantly associated with overall survival (OS), as demonstrated in a forest plot (Fig. 2C). This evidence concerns the gene MTHFD2 and breast cancer.