Reflecting the biochemical effects, treatment with T-5224 suppressed expression of CAF effector genes in the ANKRD1 overexpressing cells (Fig. 7i) as well as in CAFs (Supplementary Fig. 6d) and, in co-culture assays, was sufficient to counteract the growth-enhancing effects exerted by these cells on neighboring cancer cells (Supplementary Fig. 6e, f). Here, ANKRD1 is linked to cancer.