Finally, MEG3 NC and overexpression plasmid were co-transfected with miR-31 NC, mimic and inhibitor into CRC cells, and the detection of SFRP1 expression level indicated that MEG3 overexpression plasmid could reverse the expression of SFRP1 reduced by miR-31 mimic and synergize with miR-31 inhibitor to increase the expression of SFRP1. The gene discussed is MEG3; the disease is colorectal carcinoma.