UNC13A and Alzheimer disease: To confirm results obtained by qPCR, we performed reverse transcription PCR (RT-PCR) on several samples from each disease group using primers spanning constitutive/cryptic exon junctions for cryptic splicing events in STMN2, ELAVL3, and UNC13A; cryptic exons were detected only in LATE-NC cases but not Control or AD cases (Fig. 2i).