AKR1A1 and steatosis: Additionally, serum analysis of the liver function indexes (AST, ALT) indicated liver dysfunction in alcohol-fed Akr1a1−/− mice (Fig. 2C, D), and the results of histopathological staining further demonstrated that the loss of AKR1A1 function led to aggravated alcohol feeding-induced steatosis, fat accumulation, and fibrosis in their livers (Figs. 2G, 4D and E and 5A and B).