Interestingly, three new double missense mutations were identified by HRM assays in compound heterozygous females with G6PD deficiency, including G6PD Gaohe + Viangchan (c.95A>G, c.871G>A, c.1311C>T, and c.1365-13T>C), G6PD Valladolid + Viangchan (c.406C>T, c.871G>A, c.1311C>T, and c.1365-13T>C) and G6PD Viangchan + Canton (c.871G>A, c.1376G>T, c.1311C>T, and c.1365-13T>C). This evidence concerns the gene G6PD and G6PD deficiency.