Dietary intervention for 8 and 16 weeks significantly improved β-HAD activity in GAN DIO-NASH mice (8 weeks, 188 ± 3.0 μmol/g/min, p < 0.001; 16 weeks, 205 ± 3.8 μmol/g/min, p < 0.001; 24 weeks, 188 ± 18.5 μmol/g/min, p > 0.05), see Fig. 6B. Citrate synthase activity, a surrogate marker for mitochondrial density, was slightly but significantly lowered in the 8-week GAN DIO-NASH control group with no significant changes observed in the 16- and 24-week groups (Fig. 6B). The gene discussed is CS; the disease is metabolic dysfunction-associated steatohepatitis.