In tumor-induced skin, a single treatment with AFL monotherapy resulted in significant mean reductions of mRNA expression of all three hedgehog genes (Gli1, 72.4%, p = 0.003; Gli2, 55.2%, p = 0.010; Ptch1, 70.9, p < 0.001; Figure 4A–C and Table 1). This evidence concerns the gene PTCH1 and neoplasm.