On day 15, the distance of melanoma cells invading in gel embedded with GFP/M50 was four times greater than that of shYA–P1-GFP/M50 (1.34 ± 0.28 mm vs. 0.33 ± 0.14 mm in Fig. 4S), suggesting that CAFs require YAP1 to make the gel accessible for A375 to invade. This evidence concerns the gene YAP1 and melanoma.